Barely Significant

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The ABA content in all the treatments showed a decreasing trend; at the early embryo germination stage, the ABA content in the PEG treatment was significantly lower than in the other treatments; during the seedling germination stage, the PEG treatment was significantly lower than the control and the Na 2 CO 3 treatment ( Figure 3 C).
The expression levels of spermatogonia markers dnd1 , stmn1 and zbtb16 ; spermatocytes markers ccne2 , ccnb1 and e2f2 ; and spermatid/spermatozoa marker cfap69 were not significantly affected ( p > 0.05), even though there was an elevated trend ( Figure 5 ). 2.6. psmd9 Knockdown and Its Effect on Other Genes We further performed a knockdown of psmd9 by RNAi.
Moreover, due to the too small number of patients in the studied subgroups, the probability of using the analyzed molecules as potential biomarker molecules, useful diagnostically, showed only a small probability between individual types of ASRM and healthy volunteers, while in the case of comparisons between individual ASRM, the diagnostic test was insignificant or almost insignificant ( Supplementary Material Table S2 ).
While current guidelines recommend hormonal therapies such as combined oral contraceptives, gestagens, or GnRH agonists and antagonists [ 16 ], researchers constantly search for hormone-free alternatives, driven by a clear trend away from hormones in this particular patient collective, as well as lacking therapeutic options for women trying to conceive, yet suffering from severe endometriosis-associated symptoms, women for whom hormonal therapies are not adequate due to their contraceptive effect.