Barely Significant

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These beneficial effects were reduced following the SBW epidemic and dry conditions during the 1970s, resulting in a negative mean BAI long-term linear trend for spruce in mixed stands ( Figures 4 , 5B ) and in a positive trend for aspen in the same stands ( Figure 5H ), although aspen showed a growth decline after the mid-1980s ( Figure 4 ; Supplementary Figure 4 ).
An increasing trend was observed for TSS content at all development stages, i.e., 4.97, 7.87, 9.77, and 10.67 at 10, 18, 26, and 34 DAP ( Figure 1C ), respectively, whereas watermelon flesh’s firmness was measured as 2.72, 2.05, 1.68, and 1.27 kg/cm 2 at 10, 18, 26, and 34 DAP ( Figure 1D ), which has an opposite trend compared with fruit weight and TSS. pH can reflect the change of watermelon acidity, high pH is consistent with low acidity. pH values were recorded as 4.95, 5.14, 5.33, and 5.66 at 10, 18, 26, and 34 DAP ( Figure 1E ), respectively, indicating a decrease in acidity during fruit development.
Nine classes were obtained through the analysis of all the DAMs, among which the content of candidate metabolites in classes 1 and 7 presented an increasing trend, with 49 and 52 DAMs, respectively, such as sucrose citrulline and histidine; in contrast, 65 candidate DAMs in class 4 showed a decreasing trend, similarly, in classes 5 and 6, the metabolites also showed a downward trend during the watermelon flesh development, and there were 30 and 38 candidate DAMs, such as naringenin chalcone, vanillin, salicylic acid, and adenosine ( Figure 2D and Supplementary Table 1 ).
highly significantqualifiedgold
From these GWAS, we identified highly significant QTNs ( p -values below 10 −7 ) associated with seed weight obtained from optimal (9 QTNs) and heat stress (20 QTNs) growing conditions, as well as 2 QTNs potentially involved in plasticity ( Supplementary Figure 3 and Supplementary Table 5 ).
There is an overall trend of reduction in genetic diversity for most invasive plant species during introduction ( Dlugosch and Parker, 2008 ), although there have been rare cases of successful invaders that have shown higher levels of within population genetic diversity in the introduced range than in the native range ( Novak and Mack, 1993 ; Genton et al., 2005 ).
Concerning the micronutrients ( Figures 3E–H ) and non-essential elements ( Figures 3I,J ), the P deprivation in the NS led to a decreasing trend in all the elements in the roots of cucumber plants, except for Mn, but caused an increase in the concentration of Fe, Mn, and Ba in the aboveground tissues.