Barely Significant

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The number of Observed_ASVs increased with the increase in the number of sequences, and the curve showed a trend of gradual increase and then flattening, indicating that the sequencing data were reasonable, and the sequencing depth could reflect the species information in the samples ( Supplementary Figure S2 ).
fruticosa had a significant direct effect on understory plant community composition and a marginally significant direct effect on soil fungal community composition, and an indirectly effect on soil bacterial community composition, there was no significant correlation between nematode and understory plant and soil microbial community composition ( Figure 3D ).