Barely Significant

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“Bed Characteristics + Year” was best supported for Δ % Generalists (ΔAICc = 0.00), displaying a negative trend with D 50 . Δ % Herbivores was best modeled by “Year-only” (ΔAICc = 0.00), although the “Hydrologic Variability + Year” model was also supported (ΔAICc = 0.80). Δ % Tolerant was best modeled by the “Bed Characteristics + Year + Riparian Canopy” model (ΔAICc = 0.00), with a significant negative relationship with riparian canopy cover and a negative trend with D 50 , while the “Riparian Canopy + Year” model was also supported and showed the same negative relationship with riparian canopy cover (ΔAICc = 1.28, Table 4 ).
Microtia samples with BMP7 addition showed a trend toward greater expression of elastin, type II collagen and SOX5 compared to all other groups ( Table 3 , trendline slopes), and both BMP7-enhanced groups exhibited a decreasing trend in type III collagen gene expression when compared with their respective groups without the growth factor ( Table 3 , trendline slopes).
RT-qPCR analysis showed an increasing trend in gene expression levels of elastin, type II collagen and SOX5 in the microtia group in the presence of BMP7 compared to the other three groups, and both microtia and normal neocartilage groups with BMP7 supplementation were found with decreased type III collagen expression compared to the respective two groups without BMP7 addition.