Excerpts

Notably, our data indicated a nominally significant enrichment ( P <0.05, 1 million permutations) of credible MPB SNPs in enhancer regions from DPCs (treated with dihydrotestosterone, DHT), which further supports the hypothesis that DPCs are involved in MPB aetiology 25 (Fig. 2; Supplementary Data 1 ).

We then performed a pathway enrichment analysis on all 353 nominally significant ( p < 0.05) genes from the burden test (i.e., CVM-associated genes).

For example, consider a pathway which displays enrichment when all variants are considered ( P < 1), versus a pathway which only displays enrichment when nominally significant variants are included ( P < 0.05).

The remaining seven associations were nominally significant in the meta-analysis ( β between −0.05 and −0.07, p < 0.05) (Supplementary Data 2 ).

We then prioritized 97 CpGs and 10 DMRs by requiring these CpGs and DMRs to be also FDR-significant (i.e., FDR < 0.05) in our previous brain sample meta-analysis 5 and at least nominally significant (i.e., P- value < 0.05) in our current blood meta-analysis (Fig. 2 ).

However, 17 other covariates were nominally significant ( P -value<0.05) for HGF and 4 (weight, sample round, systolic blood pressure and age) remained significant after correction for multiple testing.

Of the 66 significant loci in the European Caucasian meta-analysis, 56 were nominally significant ( P < 0.05), and 37 after Bonferroni correction ( P < 7.6e-04) in the meta-analyzed replication cohort.

Using significant meQTLs on chromosome 1, analyses using only African American samples ( N = 112) showed that 99.91% of the meQTLs were directionally consistent with the full analysis, with 92% marginally significant ( P < 0.05) and 66% genome-wide significant (FDR < 0.01) in the smaller sample size and an overall sharing of π 1 = 0.995.

Although this analysis did not yield any significant genes after multiple testing correction, 15 genes were nominally significant ( p < 0.05), corresponding to a 2-fold enrichment with respect to the null hypothesis (Supplementary Fig. 7c ).

6 , which showed marginal significance for all three variables ( P = 0.05, 0.06, 0.05, respectively).

AEI values were significantly elevated in T21 compared to controls in the PFC ( p = 0.01, linear regression), and showed a marginal trend in the hippocampus ( p = 0.05) (Fig. 5B ).

Of the 174 lead SNPs identified in the discovery cohort, 145 (83.3%) remained nominally significant ( P < 0.05) in this replication dataset.

In the AFR samples, we found no significant effects in any of the examined p -value thresholds, although all but one were nominally significant ( p < 0.05).

Among these 38 genes, 13 replicated at a Bonferroni significance level (Logistic Regression p < 0.0013) with directions of effect consistent with the discovery findings in a cohort of unrelated, non-Hispanic white Kaiser Permanente health plan members (6653 PrCa cases, 30,121 controls), and an additional six were nominally significant ( p < 0.05; Table 1 ).

The branch‐site test showed that only estrildid FSHR orthologs had a large nonsynonymous (dN)/synonymous (dS) substitution rate ratio (branch-site dN/dS of ω » 1) that was highly significant (likelihood ratio tests [LRTs], P < 0.05) (Fig. 1b ).

We provided detailed RegulomeDB annotations in relevant cell types (e.g., hematopoietic multipotent progenitor cells, CD34+ hematopoietic progenitor cells, any primary B-cell, lymphoblastoid cell lines from 1000 Genomes [i.e., GM19238], K562, NAMALWA, BLaER1) for: (a) all index variants; and (b) credible set variants with nominally significant ( P < 0.05) functional probability scores.

Eight of the 13 ALFRED genes with a nominally significant association between RDGVs and AI in at least one cancer type in the ALFRED analysis ( P < 0.05; Fig. 2d and Supplementary Data 5 ) also had an enrichment of RDGVs in a matched cancer type compared to in controls ( P < 0.05, Fig. 3d ; Supplementary Data 6 ): ATM in colon and rectum adenocarcinoma (COADREAD), lung adenocarcinoma (LUAD) and in PRAD, NSD1 in OV, and TPCN2 in uterine corpus endometrial carcinoma (UCEC).

When AYA patients were stratified by SIL spatiotype regardless of ICI outcome, the SIL high group showed a trend of longer OS and a near-significant longer PFS ( P = 0.0504) compared to SIL low group (Supplementary Fig. 5f, g ).

5C ; P = 0.0161, Mann–Whitney test) and a marginally non-significant difference for those in complex clusters ( P = 0.0506, Mann–Whitney test).

In addition to significantly reduced paralysis scores, the Jun11695- treated group also had significantly increased weight compared to control animals ( p = 0.001 repeated measures ANOVA), and the Jun11787- treated group also had increased weight, though it was not quite significant compared to controls ( p = 0.0508, repeated measures ANOVA) (Fig. 5b ).

However, the increase was relatively moderate and only showed a statistical trend ( p = 0.0509).

Moreover, αGITR treatment, along with Treg cell depletion, also showed a trend ( p = 0.051) toward prolonged survival of GBM-bearing mice over Treg cell depletion alone (Supplementary Fig. 9b ).

The only significant interaction was between Group and Novelty (F(1,52) = 5.237, p = 0.026), showing that the DiffSt group classified old stimuli more accurately than the SameSt group (approaching significance in a post-hoc t-test, t(52) = 2.040, p = 0.051).

In correspondence with increased selfing, hoverfly-pollinated plants showed a trend towards reduction in pistil length ( Supplementary Table 2 ; P =0.051), and a significant decrease in the emission of the three scent compounds methyl salicylate, p -anisaldehyde and indole ( Table 1 ; Supplementary Table 2 and Fig. 4c ).

These 40 consensus modules were significantly related to cortisol stress reactivity in the discovery sample (multiple analysis of covariance (MANCOVA) Pillai's trace=0.72, F(40,37)=2.4, P =0.004) and borderline significant in the cross-tissue replication sample (MANCOVA Pillai's trace=0.21, F(40,212)=1.4, P =0.051).