Barely Significant

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Chlorophyll fluorescence parameters Under TR4, TR6, TR6L, and TR6S, with DAF, the actual photochemical efficiency of PSII (ΦPSII) and photochemical quenching coefficient (qp) of both NS22 and NS44 showed a trend to decline, and the decline was slow in the early period and sharp in the later period ( Figure 6 ).
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Among these, many genes are differentially expressed in A2B1, such as dihydroflavonol reductase (DFR) (A2B1 is 5 times larger than A1B1, extremely significant; 2 times larger than A3B1, significant), anthocyanidin synthase (ANS) (A2B1 is 1.6 times larger than A1B1, highly significant; 1.4 times larger than A3B1, significant), UDP-glucose: Flavonoid 3-O-glucosyltransferase (UFGT) (A2B1 is 3.8 times larger than A1B1, highly significant; 4.5 times larger than A3B1, significant).
While an elevated trend was observed in root GUS activity for some lines following heat induction, the change was not significant, indicating a lack of significant reporter gene activation in roots with the applied heat treatment ( Supplementary Figure S5 ).
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Specifically, AS26 was highly significant positively correlated with Aba Tibetan and Qiang Autonomous Prefecture (SP07) and Nagqu City (SP19); AS39 with Linzhi City (SP17); AS53 with Shigatse City (SP15) and AS55 with Hotan City (SP41), which indicated that each of the above germplasms was suitable for planting in similar regions represented by corresponding administrative regions.