Barely Significant

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An overall trend of decreased orthologs relative abundance from Day 0 to Day 10 was similar in the S2 + BB12 group, except for four orthologs that had a non-statistically significant increase following BB12 administration, including fliC (flagellin), msbA ; ATP-binding cassette, subfamily B, bacterial MsbA, sstT ; serine/threonine transporter, and chondroitin-sulfate-ABC endolyase/exolyase ( Figure 2J ; Supplementary Table S1 ).
We found a clear trend of segregation of significantly different genera between the two groups, with stronger aggregation of significantly higher genera (green nodes) in the rumen of adult buffaloes and stronger aggregation among significantly higher genera (yellow nodes) in the rumen of breastfed calves, suggesting that we have a community effect and symbiotic relationship between microorganisms in the rumen of buffaloes at different growth stages ( Figure 4 ).
Acetate, butyrate, and propionate exhibited an increasing trend from the beginning of every stage to the end of the same stage, but the gradient or the rate of production so to speak of acetate and propionate were significantly diminished compared to their respective times at the end of every stage (170 mM (48 h) > 134 mM (96 h) > 121 mM (144 h); 76 mM (48 h) > 42 mM (96 h) > 23 mM (144 h), whereas for butyrate, the opposite was observed, (69 mM (48 h) < 117 mM (96 h) < 176 mM (144 h).