Barely Significant

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The α-diversity analysis revealed an increasing trend in the Chao 1 index for the T1 and T2 groups ( Figure 3 B), though this was not significant, while the Shannon index significantly decreased in T2 compared to that in CK ( Figure 3 C), suggesting that the EC-520 intervention had no substantial impact on the richness of the microbes in the rats’ colons, while indicating a slight decrease in bacterial diversity.