Barely Significant

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At the genus level, the relative abundance of norank_f__Muribaculaceae , Lachnospiraceae_NK4A136_group , Alistipe, Parabacteroides , Bacteroides , and Prevotellaceae_ UCG-001 in the CTX group showed a decreasing trend, while the abundance of Lactobacillus and unclassified_f__Lachnospiraceae significantly increased.
Among them, the ROAV of 1-octen-3-ol gradually decreased with the extension of cooking time, and the ROAV of 2-pentylfuran and methyl cinnamate showed an increasing trend, which indicates that long-term cooking resulted in loss of the mushroom aroma of T. matsutake soup, but it would bring some floral and fruity aroma.
The α-diversity analysis revealed an increasing trend in the Chao 1 index for the T1 and T2 groups ( Figure 3 B), though this was not significant, while the Shannon index significantly decreased in T2 compared to that in CK ( Figure 3 C), suggesting that the EC-520 intervention had no substantial impact on the richness of the microbes in the rats’ colons, while indicating a slight decrease in bacterial diversity.
Using whole wheat bread as a partial malt substitute in brewing did not result in significant changes in turbidity, except for hazy beers like Bavarian weiss styles, where a decreasing trend and significant differences were observed when malt was replaced by whole wheat bread versus the control.
Meanwhile, the transcriptomic analysis of the glutathione metabolism pathway in beef samples revealed that, compared to Qinchuan cattle and Simmental cattle, Pingliang Red Cattle samples had eight genes with extremely significant upregulation in their relative expression levels, and these expression levels were positively correlated with the glutathione content in the beef ( Figure 8 b).