Excerpts

Candidate gene analysis Our Gene Ontology pathway enrichment analysis of the impacted genes within the CNV breakpoints identified “detection of chemical stimulus involved in sensory perception (GO:0050907)” and “nervous system process” (GO:0050877) pathways to be highly significant (FDR P < 2.1 × 10 –6 ) and (FDR P < 9.0 × 10 –3 ) after correction.

Escape latency time in spatial task learning The effects of factors “treatment” and “day” are highly significant in two‐way ANOVA analysis: F 4,25 = 15.07, p = 2.14 × 10 −6 and F 3,75 = 20.38, p = 9.26 × 10 −10 respectively.

As shown in Figure 3 the mutated promoter fragment resulted in a statistically highly significant ( P =2.2×10 −6 ) reduction of the relative response ratio to approximately 34.6% of the wildtype promoter in the presence of C/EBP (+ C/EBP).

As this test was extremely significant (p-value = 2.2*10 −6 ), we rejected the null hypothesis and assessed the effect of the partial smoke-free legislation on AMI incidence, mortality, and case-fatality by negative binomial regression analysis.

Even when we excluded Actinomycetota, which make up a large proportion of genomes that do not encode the motif, this finding was still highly significant ( P = 2.2e −06 ) ( Fig. 6B ).

Even with those genes excluded, correlating the d N / d S and number of separate recombinant sequence regions identified per gene in the pan-genome with a nonparametric Spearman's rank correlation returned a highly significant negative correlation coefficient of −0.122, with P = 2.21 × 10 −6 .

Results indicated highly significant differences ( P = 2.24 × 10 −6 ) attributed only to the inoculum pretreatment (Fig.

Using the MLM method, we were only able to identify the marginally significant association for ZmCCT (−log 10 ( P ) = 5.64) and there were no strong signals in other genome regions ( Figure 2B ).

This difference was highly significant ( p = 0.0000023; Figure 11b ).

Another closely significant SNP was located at scaffold43:154,009 ( P = 2.3 × 10 − 6 ) within the SHROOM2 gene.

At a minimum length of 3 Mb, the rate in cases and controls dropped to 0.65 and 0.52, respectively, indicating a highly significant excess of homozygosity in cases (ratio: 1.25, p = 2.30×10 −6 ).

A cross-tabulation analysis revealed a highly significant enrichment of HPV16-positive OPSCC for the SC low group, which was almost absent in the SC high group ( p = 2.33 × 10 −6 ).

40 Because the association of TGFβ as an upstream regulator was highly significant ( P = 2.33 × 10 – 6 ) and all differentially expressed genes were engaged in the TGFβ pathway, we decided to further study the role of TGFβ on these mechanoresponsive glaucoma-related genes in bovine AAP cells first, followed by validation in human SC cells.

Statistical analysis revealed that the difference in RNA yields between the KingFisher semi-automatic method and the manual method was highly significant ( p = 2.36 × 10 −6 ).

For instance, two of the nodes/cluster which appeared to be highly significant based on the correlated p value and the high number of gene members were found to be “root system development” ( x = 12/ n = 230, p value = 2.36E−06) and “root‐development” ( x = 12/ n = 230, p value = 2.36E−06).

This improvement of 16.0% points was highly significant (paired t-test, P = 2.4 × 10−6).

For the OC cultures obtained with the standard procedure the number of monocytes, neutrophils and lymphocytes in peripheral blood was not significantly different in respect of the OCs obtained, whereas there was a highly significant difference ( p = 2.4×10 −6 ) respect to the number of recovered monocytes, suggesting that OC number was proportional to the recovered monocytes and directly dependent on the number of OC precursors isolated (Table 2 ).

The results were consistent with the preliminary observations from Figure 5 A: there was a significant correlation between the core genes and rare genes with the λ parameter, with a highly significant strong negative correlation between the core genes and λ ( p -value < 2.44 × 10 −6 and a correlation coefficient of −0.910, and the explanatory power of the linear model R-squared reached 0.829).

A highly significant decrease [F(1,24) = 37.61, p = 2.46E-06] was observed (Figure 3B ) when the average Aβ 1-42 level of the nasal IgG1-iS18 antibody treated mice (Mean = 227.71 pg/mg, SD = 15.06 pg/mg) was compared with that of the PBS treated mice (Mean = 370.21 pg/mg, SD = 82.41 pg/mg).

All models that estimated heritability for BMI, weight, and height were highly significant for rejecting the null hypothesis of h 2 = 0, with p -values < 0.0000025.

SNPs with marked allele frequency differences between wild and domestic chicken showed a highly significant deficiency in the proportion of amino-acid altering mutations (P<2.5×10 −6 ).

Strikingly, Pita program [39] predicted a highly significant global miRNA binding preference to the reference genomic sequence over the mutated sequence (p = 2.5×10 −6 ) in the melanoma patient genome, independently of the exact ΔΔG threshold chosen to define binding ( Figure 1A ).

In contrast, there was a significant trend ( P = 2.5 × 10 -06 ) for increases in HGF mRNA levels in CD138+ plasma cells, with progressive severity of disease from healthy donors to patients with relapsed or refractory MM (Figure 1 B).

In contrast, fungal infection showed a decreasing trend across groups (0.066, 0.045, 0.035, 0.051; p = 2.51 × 10 −6 ), consistent with its inverse association with HPV infection.

This is highly significant assuming an additive model t = 5.2 (Student’s left-tailed t-distribution p = 2.51 x 10 −06 ), with no evidence for a dominant effect (dom = 0.12 ± 0.09) t = 1.25 (Student’s left-tailed t-distribution p = 0.18).