Barely Significant

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From the proteome perspective, the distribution of evolutionary ages of expressed (detected) versus non-expressed (undetected) proteins exhibited a clear trend (Fig. 3a ): expressed proteins represented a dominant fraction (70–80%) in the oldest phylostrata (ps1-4), and their percentage then continually dropped, to reach zero in some of the most recent phylostrata (ps13 and ps15).
In the pH-sensitive luciferases and their mutants, although the calculation of the overall microenvironment charge, considering the four residues H310, E311, R337 and E354, showed a trend for blue-shifted emission in electrostatically more negative environments and red-shifted emission for more positive environments (Table 2 ), the mutant R337E, in which there is a substantial increase of the overall negative charge character of the environment, also resulted in red light.