Barely Significant

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highly significantqualifiedgold
c regression multivariate equation was established as follows: Y = 21.25 + 0.798333A − 0.0258333B + 1.13333C + 1.06917D + 0.8125AB − 1.035AC − 0.3475AD − 0.2375BC − 0.0025BD − 0.7075CD − 1.19292A 2 − 2.25417B 2 − 2.72792C 2 − 1.05417D 2 As shown in Table 2 , the model exhibited a p -value < 0.0001 (highly significant), while the lack-of-fit term had a p -value of 0.5375 (not significant).
In some cases, an increasing trend for a given constituent was observed compared with its content in the initial material; for instance, the isomenthol content in the initial material was 0.33%, while in the oil isolated after glycerol–water extraction (65%) the contribution of this constituent was 0.48–0.57%, depending on the applied time and temperature conditions.
Among sirtuin family members, SIRT1, SIRT3, and SIRT4 exhibited >2-fold induction (log 2 FC ≈ 1.0–1.3), whereas SIRT5 showed a positive trend that did not reach statistical significance, likely due to low read counts and partial RNA degradation in irradiated samples.
Overall, freeze-dried (FzD) habanero pepper leaf samples extracted with ChCl exhibited the highest TPC values, although these showed a decreasing trend as extraction time increased, whereas oven-dried (OvD) samples obtained with the same HBA showed the second-highest TPC values, with a linear increase as extraction time increased.