Barely Significant

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The relative abundance of Thaumarchaeota , Candidatus Bathyarchaeota, Ascomycota, Basidiomycota, Chlamydiae, Apicomplexa , and Candidatus Brennerbacteria showed an increasing trend followed by a decreasing trend with increasing N deposition, with the highest relative abundance observed in the N30 treatment ( Table 2 ).
After treatment with HS/HSE, the proportion of Firmicutes increased in the HS group, and interestingly, the number of Bacteroides also showed a trend toward increasing in the HS group compared with the MC group, but the Firmicutes/Bacteroides ratio in the HS group was almost equal to that in the MC group.
Independent measurements of the derivative reagent δ 2 H values are possible in some cases, but without accompanying correction for the amine-bound hydrogen in derivatized amino acids, errors in the reported δ 2 H values of amino acid carbon-bound hydrogen may be significant (on the order of 10 to 100‰; Supplementary Figure S7 ; Supplementary Section 4 ).
Alpha diversity analysis of soil microorganisms at different elevations in the early freeze–thaw period of the Qinghai Lake watershed ( Figure 2B ) showed that the species richness indices of microorganisms (ACE, Chao1, observed) showed an increasing trend with the increase in elevation.