Barely Significant

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highly significantqualifiedgold
Patients with excess blasts (RAEB1/2) tended to have lower AMC (median 0.19 versus 0.32 for patients without excess blasts, p < 0.0001) and lower AMC were found in higher IPSS-R categories (very low, 0.37 × 109/L; low, 0.30 × 109/L; intermediate, 0.25 × 109/L; high, 0.16 × 109/L; very high, 0.20 × 109/L), while there was a highly significant difference in the AMC between higher risk (intermediate, high, very high) and lower risk (very low and low) MDS according to the IPSS-R (0.21 × 10 9 /L vs 0.33 × 10 9 /L, p < 0.0001).
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After processing the data using the Seurat pipeline and analyzing the single-cell expression data, gene set enrichment identified Epithelial gene expression, Cigarette smoking , Airway epithelium , and Chronic obstructive pulmonary disease as the top gene modules with highly significant p-values, confirming that smoking-related pathways were correctly predicted using E.PAGE (Table 7 ).
Given that a previous study identified a near-significant association at the CFA10 locus in a case–control GWAS within the ACS breed 12 , we further investigated the genotypes at the CFA10 SNP in the present ACS population—85.7% of affected dogs were homozygous for the A allele compared to 62.5% of controls (Supplementary Table S1 ).