Excerpts

Although in this study a lack of association due to the low frequency of the variants was observed for 1007finsC and G908R polymorphisms 32 , a strong trend ( p = 0.05) towards a reduced presence of the mutated R702W allele was observed in IA patients 32 .

All signals show at least nominally significant ( P <0.05) directionally concordant associations in the follow-up studies of clinically validated PCOS cases, with no significant heterogeneity by PCOS case definition ( Supplementary Table 2 ).

Of these, 22,296 were nominally significant ( p < 0.05) and 4192 were significant to a genome-wide Bonferroni level ( p < 1 × 10 −7 , linear mixed model) for GWAS risk haplotype-capturing SNP allelic count.

We performed immunoprecipitation-mass spectrometry (IP-MS) identification of N-terminal-tagged CLK2-associated proteins (4 FLAG-tagged replicates, one untagged CLK2), using tandem mass tags (TMT) mass spectrometry and observed highly significant enrichment (Fig. 6a , Supplementary Data 32 ) of both known CLK-interacting proteins (28/97 known, B-H adjusted Wald p < 0.05) including CLK1 and additional splicing factors (98/270 total enriched, B-H adjusted Wald p < 0.05).

The minimum sample size requirement is based on experience and the fact that the observed differences between different conditions were statistically highly significant ( p <<0.05, Figs. 1 e, 2 d, 4 c, 7b ) and never at the borderline value of p = 0.05.

After mapping these CpG sites to genes, performing GO enrichment analyses, and removing overlapping GO terms, there were a total 95, 76, and 47 nominally significant (hypergeometric test P < 0.05) biological processes, respectively.

We defined a variant’s eGFRcrea association as validated by eGFRcys/BUN when we observed a directionally consistent, nominally significant association with eGFRcys and/or BUN ( P < 0.05, same effect direction for eGFRcys and/or opposite effect direction for BUN).

In total, 63 loci have shown at least marginal significance ( P <0.05) with ER-negative breast cancer.

Of note, we observed a borderline significant decrease in phosphorylated eIF2α levels in PKR KO animals (two-way analysis of variance (ANOVA), genotype main effect P =0.05), suggesting that PKR indeed contributes to eIF2α phosphorylation status in adipose tissue, irrespective of dietary status ( Fig. 4h and Supplementary Fig. 4 ).

ClinVar data were downloaded as VCF (accessed 02.05.2022) and filtered for nominally significant single variants ( P < 0.05 in any phenotype model).

Table 2 provides a list of the strongest associations identified across the entire data set, all highly significant (Bonferroni-corrected P <0.05).

There was a marginally significant effect of group on absolute value rating difference (between-subjects Kruskal–Wallis test: H 3 =7.74, P= 0.05; Fig. 5a ).

Embryos treated with (5Z)-7-Oxozeaenol showed a slight, but not quite significant (p = 0.05, Student’s t-test) increase in the percentage of cells expressing cleaved Caspase 3, suggesting that in these embryos another mechanism may be responsible for the decrease in extra-embryonic cells.

However, 17 other covariates were nominally significant ( P -value<0.05) for HGF and 4 (weight, sample round, systolic blood pressure and age) remained significant after correction for multiple testing.

In subgroup analysis, the six VTE risk genes were nominally significant ( P < 0.05) in both female and male groups and showed the same effect directions as in the full sample (Supplementary Fig. 2 ).

After filtering to the 176 clock-disease associations that were Bonferroni significant in the Cox models, there were 32 instances where the AUC improvement between the null and full model was greater than 0.01 and nominally significant at P < 0.05 (Fig. 2 and Supplementary Data 8 ).

Univariate linkage disequilibrium score regression (LDSC) 24 analysis showed nominally significant heritability for all 37 phenotypes ( P < 0.05, Table 1 ).

Among 15 associations with more than two instrumental variables (9 metabolites, 7 FinnGen outcomes), seven associations (3 metabolites, 6 outcomes) remained nominally significant ( P -value < 0.05) in MRPRESSO outlier test.

The branch‐site test showed that only estrildid FSHR orthologs had a large nonsynonymous (dN)/synonymous (dS) substitution rate ratio (branch-site dN/dS of ω » 1) that was highly significant (likelihood ratio tests [LRTs], P < 0.05) (Fig. 1b ).

According to BADGERS, the reference panel comprises 1738 traits from the UKB 17 , selected on the basis of nominally significant SNP-based heritability ( P < 0.05).

3c ), and immune scores showed a trend and modest correlation with PFS (Spearman ρ = 0.35, p = 0.05; Fig. 3d ).

coli lysates expressing IsmA and SpiR revealed that SpiR generated a higher cholestenone signal, with a marginally significant difference ( U = 9.0, p = 0.05, Mann–Whitney U test).

While integrative analysis of pathways restored by the microbiota-targeted interventions did not yield any FDR-significant results in either sex, several nominally significant pathways ( p- value < 0.05) were observed, highlighting potential effects on developmental, signaling, and metabolic processes.

We found 99.3% (5442/5478) of the FDR significant associations also had a nominally significant ( p ≤ 0.05) association in the Spearman rank test (Fig.

We then determined the time series of β SOT using 15-year moving windows from 1982 to 2021 at each pixel and found that 38.6% of regions exhibited a significant increase in β SOT for kNDVI, while 22.0% showed a decreasing trend ( p < 0.05) (Fig. 1A ).