Barely Significant

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Regarding Na + ( Figure 6C ), its soil content exhibited a decreasing trend regardless of treatment with either C. sorokiniana or the Bacillus combination, with reductions of 52.03% and 5.86%, respectively. 3.6 Effects of C. sorokiniana and Bacillus combination on soil microbial diversity and community function The alpha diversity indices of bacterial communities in soils subjected to different treatments are shown in the Figure 7 .
The expression level of the SPS1 was elevated with advancing growth stages at density D2, while SPS2 exhibited the opposite expression pattern; IVR1 and IVR2 showed little change in expression levels at different densities as the growth stage progressed in M1, but expression levels were elevated as the growth stage progressed at density D2 in M2; and BAM1 and Sh2 showed a decreasing trend in expression levels as the growth stage progressed and the stalk gradually aged.
In recent years, with the continuous expansion of protected tomato cultivation area, the intensification of continuous cropping obstacles, and the frequent cross-regional circulation of seedlings, the occurrence of tomato viral diseases has shown an increasing trend year by year, among which tomato yellow leaf curl virus disease is one of the most serious viral diseases ( Wang et al., 2025a ).TYLCV belongs to the genus Begomovirus in the family Geminiviridae, mainly transmitted by whiteflies (Bemisia tabaci) in a persistent manner.
Although the high level of nitrogen fertilizer N3 (450 kg·hm -2 ) helped to increase the soil respiration rate, the increase in respiration rate of N2(300 kg·hm -2 ) and N3(450 kg·hm -2 ) treatments stabilised in 2023 and there was a decreasing trend in soil respiration rate under N3(450 kg·hm -2 ) treatment ( Figure 7c ). 3.2 Soil chemical properties 3.2.1 Organic carbon and N content Aerated irrigation combined with nitrogen fertilizer application significantly increased the soil organic carbon content in the tillage layer (0–40 cm), and this effect became more pronounced with the number of treatment years and the nitrogen application rate ( Figure 8a ).