Barely Significant

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Specifically, certain transcription factors (TFs) involved in chlorophyll biosynthesis exhibited a tendency to be downregulated, while others related to chlorophyll degradation showed a trend of upregulation ( Figure 2 ). 3.3. qRT-PCR verification of the genes involved in chlorophyll metabolism To validate the reliability of RNA-Seq data, twelve differentially expressed genes (DEGs) involved in chlorophyll metabolism were analyzed using qRT-PCR.
highly significantqualifiedgold
The BBD test results showed that cone height, seed storage tube diameter and seed storage tube lifting height had highly significant effects on the variation coefficients of seed distributing uniformity in the three situations that the seed distributor were horizontally tilted at 0° vertically tilted at 0°, horizontally tilted at 5° vertically tilted at 0°, and horizontally tilted at 5° vertically tilted at 5°.
Among the dynamic changes in the expression of these nine key enzyme genes ( Figure 8 ), the expression of three genes, LOC105054663, LOC105036364, and LOC105048473 (encoding the enzyme F3H, CHS, and DFR), showed a decreasing trend from FS1-FS3., This trend aligned with the changes in the levels of metabolites such as Afzelechin, Quercetin-3 -O-sambubioside*, and Baimaside.
Results of InVEST model As shown in Supplementary Table S8 , the cropland area first decreased in 2002, and then showed a trend of continuous increase, the forest area continued to increase, the shrub area fluctuated, the grassland area continued to decrease, and the water and impervious area increased.