Barely Significant

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4 Discussion 4.1 Change characteristics of vegetation and meteorological factors and their relationship with yield Through the analysis of spectral reflectance frequency curves ( Figure 3 ), winter wheat exhibits an overall trend of “ Spectral compression and peak migration” across the three growth stages, reflecting the phasic changes in spectral response to vegetation growth.
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To further elucidate the biological functions enriched among genes with significant genetic differentiation ( F ST ) between the GL wild and HTX ancient seedling populations, KEGG pathway enrichment analysis ( Figure 6C ) revealed highly significant enrichment in pathways including galactose metabolism, starch and sucrose metabolism, the spliceosome, ubiquitin-mediated proteolysis, nucleocytoplasmic transport, glycolysis/gluconeogenesis, purine metabolism, monobactam biosynthesis, and selenocompound metabolism (detailed results of the KEGG enrichment analysis are provided in Supplementary Table 5 ). 4.
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Twenty-one module-metabolite pairs exhibited correlation coefficients > 0.9 or < -0.9 ( P < 0.01), of which the turquoise module displayed highly significant positive or negative associations with five distinct metabolites, and the brown and black modules each correlated strongly with four metabolites.
Similarly, when different concentrations of L-tryptophan, L-phenylalanine, indole and 2-methoxycarbonylphenyl beta-D-glucopyranoside were exogenously added to the culture broth of hydroponically grown tea plants, there was also an increasing trend observed in root length, dry weight, net photosynthetic rate of tea plants and tea polyphenols, caffeine, and free amino acids (excluding theanine) contents of tea leaves.
It can be seen from this that the distribution range of the occurrence frequency of compound hot extremes and droughts in Northwest and Southwest China is relatively wide, indicating that there may be significant differences in rainfall and high-temperature drought risks at different locations within these regions. 3.3.
Furthermore, in maize and sorghum, the number of BBXs in maize showed an increasing trend, while the number of BBXs in sorghum continued to decrease; the number of BBXs in Setaria species (foxtail millet and green millet) decreased to 16 and 17 ( Supplementary Figure 2 ).
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The qRT-PCR results showed a highly significant positive correlation with the RNA-seq data ( Supplementary Figure 3 ; Supplementary Table 2 ). 3.5 Integrated multi-evidence analysis identifies TraesCS6B03G1211800 as the causal candidate To robustly prioritize the causal gene from the 25 candidates and mitigate the risk of false negatives from expression screening alone, we employed a multi-layered evidence integration strategy.
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In the whole panel of wheat genotypes, a significant and positive correlation was observed for β-glucan with arabinoxylan (r = 0.52), D-xylose (r = 0.52), and protein (r = 0.58), whereas with starch and thousand-grain weight, β-glucan shows a highly significant and negative correlation (r = −0.63 and r = −0.51, respectively) ( Figure 3A ).