Barely Significant

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Compared with the original contaminated soil, the Chao1 index showed a downward trend, the species richness decreased, and the Shannon index also showed a decreasing trend after Step2 and Step3, it can be argued reduced microbial diversity in soils.
The concentrations of acetic acid, propionic acid, butyric acid, valeric acid and total acid in the CS10% and CS15% groups showed an increasing trend, but the difference was not statistically significant (0.05 < P < 0.10), and the concentrations of acetic acid, propionic acid and total acid showed a quadratic increase with the increase of dietary CS supplemental level ( P < 0.01 and P < 0.05). 3.2 Effects of chili straw on abundance, diversity, and composition of rumen bacteria 3.2.1 Venn diagram analysis of rumen bacteria We sequenced each sample group, and the coverage rates for the resulting data all exceeding 99%, ensuring that the samples collected accurately reflected the changes in the rumen bacterial community.
This is evidenced by an increasing trend in βNTI values along the symbiotic-to-saprophytic continuum, supporting our hypothesis that the filtration systems within C . camphora leaves impose significant selective pressure on microbial communities, impacting assembly even during the invasion by exogenous species in the newly fallen leaf stage.