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Vimentin expression showed a trend toward increased levels in the mucosa of mock‐treated progeroid mice, though this did not reach statistical significance (Figure S6A,B ). α‐SMA staining revealed no evidence of myofibroblast activation, indicating an early fibrotic stage (Figure S6A ). qPCR analysis of plasminogen activator inhibitor‐1 (Pai‐1) and Tgfb1 in total colonic extracts indicated a trend toward increased expression in mock Lmna G609G/G609G mice, though these differences did not reach statistical significance (Figure 3D ).
Confirming the latter observation, we detected significant higher frequencies of Cd11b + Ly6G – Lys6C + monocytes in the livers of Tgr5 −/− compared to WT littermates ( Figures 2 E and S2 A), while frequencies of neutrophils (Cd11b + Ly6G + Lys6C − ) in the livers of Tgr5 −/− mice showed a trend toward upregulation but did not reach statistical significance ( Figure S2 B).
This was accompanied by augmented mRNA levels of Cdkn1a and kidney injury marker Havcr1 in the kidneys of EC-KO vehicle-treated mice, which showed a trend of down-regulation after treatment with SUL-138 without reaching statistical significance ( Figure 4F–G ). mRNA expression of kidney injury marker Lcn2 and fibrosis marker Col1a1 showed a trend of being increased in EC-KO vehicle-treated mice without reaching statistical significance.
The glial per knockout ( repo-gal4 > per CRISPR ) significantly reduced survival to starvation in two out of three experiments and the third experiment also showed a trend toward reduced survival ( Figure 5a and Suppl.
Regulation of Metabolic Rhythms by Glial Clocks.
J Biol Rhythms · 2026 · PMC12694984