Barely Significant

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highly significantp < .0010.0× alphaqualifiedgold
However, we observed a highly significant difference in the duration of the experiment after controlling for age (ANCOVA: F(2,89) = 9.489, p < .001, mean 2-AFC = 206.20 sec, mean 4-AFC = 135.37 sec, Fig 8 ), indicating completing 30 trials in a 4-AFC task takes considerably less time than completing 60 trials in a 2-AFC task even though the average trial duration in the 4-AFC was significantly longer than in the 2-AFC experiment (mean 2-AFC = 3.44 sec, mean 4-AFC = 4.51 sec, ANCOVA: F(2,89) = 4.682, p = .01).
highly significantp<0.0010.0× alphaqualifiedgold
Finally, there was a highly significant difference in predation between the two sites ( site 1a and site 1b ) (gall predation: negative binomial GLMM, estimate = −2.05, SE = 0.44, z = −4.64, p<0.001; the incidence of gall predation: binomial GLMM, estimate = −2.25, SE = 0.31, z = −7.26, p<0.001; predation rate: quasibinomial GLMM, estimate = −2.13, SE = 0.26, df = 521, t = −8.30, p<0.001).
highly significantP value ≤ 0.0010.0× alphaqualifiedgold
The chemical-genetic interaction of the positive control methyl methanesulfonate (MMS), a well-characterized antifungal agent that damages DNA [ 28 ], demonstrated a highly significant enrichment ( P value ≤ 0.001) for cellular response to DNA damage stimulus, confirming a successful HIP-HOP assay procedure and accurate post-sequencing data analysis.