In addition, the differences between RTs for both phase ranges were highly significant (monkey B: 45.0 ms, n = 16, p = 0.0021483, t = 3.3579; monkey T: 48.9 ms, n = 18, p = 0.011919, t = 2.6571; paired Student’s t -tests, two-sided) and this effect was equally strong for RTs following the reappearance of the initial shape at the end of MCs 2 and 3 (Supplementary Fig. 5 ).
Excerpts
In the EUR GWAS for Aβ, we found that rs76490923 had a marginally significant association (beta = −0.1240, SE = 0.0404, p = 2.20 × 10 −3 ) with Aβ in EUR population. rs76490923 was associated with decreased Aβ levels and exhibited the same effect directionality (beta coefficient, −0.1240 [EUR] vs. −0.1661 [EAS]) as in our EAS GWAS results (Fig. 2 ).
This decrease was highly significant in the naive group (N-peptide, p = 0.0022, R-, p = <0.0003, and C-, p = 0.0367) whereas it was non-significant in the semi-immune group (N-peptide, p = 0.902, R- p = 0.263, and C-, p = 0.371) (Fig. 4 ).
11 , where P < 0.05 is nominally significant; specific area significance (i.e., Specific brain thickness P < (0.05)/(11 traits*2 SNPs) = P < 0.0023.
In five of these six studies, p.E40K was associated with lower fasting glucose, and nominally significant association with fasting glucose was observed in meta-analysis of these six studies ( p = 0.0024, total n = 120,050).
The results demonstrated that MX2 displayed the most significant eQTL with rs398206 ( P = 6.6e−15; linear regression), while none of the other genes in the TAD displayed even a marginally significant eQTL after adjusting for multiple testing (Bonferroni-corrected cutoff at P < 0.0024 for 21 genes; Supplementary Table 9 ).
This difference was nominally significant ( P =2.6 × 10 −3 , two-tailed Fisher's exact test, OR=6.7, 95% CI=1.7–31.1) but did not withstand multiple testing correction for all 189 candidate genes.
The most strongly associated SNP (rs804279) was intergenic, approached genome-wide significance in the Stage 1 Discovery GWAS (A allele, OR=0.74, 0.66–0.83 95%CI, logistic regression P =1.4 × 10 −6 ), and was nominally significant in the Stage 2 Metabochip Replication (A allele, OR=0.82, 0.73–0.93 95%CI, logistic regression P =2.7 × 10 −3 ) for a sample-size weighted two-strata meta-analysis P meta =1.9 × 10 −8 .
Detailed analysis of the palmitoylomics results revealed that knockdown of ZDHHC21 provoked a strong (approximately four-fold change plotted in log 2 scale, which correspond to approximately 15 times decrease in the palmitoylation level) and highly significant ( P = 0.0028) decrease of 5-HT1AR palmitoylation in comparison to the scr samples (Fig. 5h ).
The differences in slope at higher I for the low R region of the PFR are highly significant ( t (75) = 3.23, p = 0.0028).
Interestingly, treatment with concanamycin A led to a moderate but highly significant recovery of PTP induced by pre-postsynaptic AP pairing (267.5 ± 40.5%; 246.8 ± 62.5 pA in control versus 554.2 ± 112.3 pA after HFS 100 ; P = 0.003; 12 pairs; Fig. 4g–i ).
The total number of pseudogenes exhibits a moderately significant correlation with genome size (Spearman’s ρ = 0.57, p -value = 0.003) and the number of retrotransposons in the genome (Spearman’s ρ = 0.59, p -value = 0.02).
The difference in OS between the matching patients and our cohort is highly significant (median overall survival 22.7 months vs 31.1 months, P = 0.0032, Fig. 2 ).
The significant enrichment of the early clonal driver GATA3 in the Nigerian group and a positive trend in its recurrence with African ancestry (proportion trend test, P = 0.0035) along with a significantly lower age at diagnosis in patients with tumors carrying GATA3 mutations is likely to be an underlying genomic event associated with young onset breast cancer.
We detected nominally significant enrichment for islet eQTLs for variants associated with continuous glycemic traits (normalized enrichment score (NES) = 1.27; P = 3.7 × 10 −3 ; Supplementary Fig. 9 and Supplementary Data 14 ): of all the tissues considered, islets generated the most significant enrichment for this phenotype.
The usage of ART was moderately significant ( P =3.86 × 10 −3 ), with 4.25 more DNMs on average compared with natural conception when controlling for other variables.
Intriguingly, we identified a correlation between rs1800734 and DCLK3 expression in the healthy ( P =0.029, linear regression model) and tumour ( P =0.031, linear regression model) samples, and this association was highly significant in the MSS patients ( P =0.004, linear regression model) ( Fig. 2e ), indicating this locus may act as a distal enhancer and regulate DCLK3 .
Although the abundance of Clostridium was not significantly correlated with the TUDCA or NorDCA concentration ( R = −0.3141, P = 0.3734 and R = 0.3482, P = 0.3194, respectively), a highly significant negative correlation was found between the abundance of Clostridium and the primary bile acid CDCA ( R = −0.8398, P = 0.0040) (Fig. 4c ).
Second, we compare the 65 genes closest to the published GWAS T2D loci with our 3,597 T2D-DMR genes, and find highly significant overlap of genes (Fisher’s test P =0.004).
In contrast, genes near TET2 -associated sites showed nominally significant depletion for HSC marker genes (OR = 0.46; P = 0.004), though were enriched for marker genesets for naïve T cells, monocytes, and neutrophils (1 × 10 −14 < P < 0.0008; Supplementary Fig. 9 ).
We did observe inverse correlations within the female and male groups between testosterone and IgG3 levels, and oestradiol and IgG3 levels, but these correlations did not reach statistical significance after Bonferroni multiplicity correction (threshold p < 0.004) (Supplementary Fig. 8a–g ).
Furthermore, the average CRT of the followers of a given account is a highly significant predictor of which community that account belongs to (logistic regression predicting membership in cluster 2, odds ratio (OR) = 0.545, p = 0.004), such that a one standard deviation decrease in followers’ average CRT score is associated with an 83.5% increase in the odds of an account being in the low CRT cluster.
After normalizing for the difference in sequence context between the datatypes, we found a difference in the proportion of a 5′ CCG 3′ → 5′CTG 3′ mutational signature that was nominally significant in our South Asian ancestry study compared to those from the European studies (ratio 1.35, p = 0.0044) (Fig. 3b ).
Regarding the 1000 Genomes Project global subjects, the SDS-identified top SNPs in ADH clusters and BRAP-ALDH2 showed nominally significant excess of DAF heterogeneity ( P < 0.0044 for DAF heterogeneity enrichment test; Table 1 and Fig. 4a ).
We also found a highly significant enrichment (adjusted P = 4.5 × 10 −3 ) for the top-level mammalian PO term hearing/vestibular/ear phenotype (MP:0005377).