Barely Significant

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According to the growth rate hypothesis ( Harris, 2003 ; Niklas and Cobb, 2005 ), as the relative growth rate increased, N:P showed a decreasing trend and P showed an increasing trend, which indicated that the growth rate of the Calligonum species was generally the highest in the three reproductive phases.
In terms of ABs at different reproductive stages, except for the ABs of CK, which showed a trend of increasing first and then decreasing, the other three species showed significantly lower N:P in the flowering phase (an average of 8.112) and as the reproductive process progressed, there was a significant increase in the N:P ratio by the ripe-fruit phase, with the ABs of these three species averaging an N:P of 13.056.
The relative expression levels of the genes encoding farnesyl-diphosphate farnesyltransferase ( FDFT1 ), Δ14-sterol reductase ( TM7SF2 ), cholestenol Δ-isomerase ( EBP ) and cycloartenol synthase ( CAS1 ) related to steroidal saponin biosynthesis showed a trend of first increasing and then decreasing across the four growth stages.
We hypothesized that (1) there may be significant differences in the ecosystem N, P, and K cycling characteristics due to the heterogeneous effects of nutrient elements; (2) whole-tree harvesting would have a negative impact on the ecosystem N, P, and K cycling functions because whole-tree harvesting causes substantial nutrient loss; (3) whole-tree harvesting may regulate nutrient cycling functions differently due to the significant disturbances it causes to vegetation, soil, and the overall environment. 2.