Barely Significant

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The relative expression trends of the Clcsm gene ( Cla97C06G126680 ) and ClMYB36 gene ( Cla97C10G203210 ) were highly significant in the clear stripe margin pattern line (slb, P 1 ) than the blurred stripe margin pattern line (GWAS-38, P 2 ); the ClAPRR5 gene ( Cla97C01G000070 ) showed highly significant expression in the blurred stripe margin pattern line (GWAS-38, P 2 ); the other seven genes ( ClMYB20 , ClMYB10 , ClRAX3 , ClIPN2 , ClEFM , ClAPRR2 , and ClKAN2 ) did not show any significant differences in expression level between the comparative parental lines.
Subsequently, with the increase of calcium concentration, the TC, TN, TP, and TK contents in each organ and the whole plant showed a decreasing trend, and when the calcium concentration reached 400 mg·kg -1 , they all significantly decreased compared to the control without calcium (except for the TP content in stems) ( p < 0.05).
The changing trends of C:N and C:P ratios in Pinus tabuliformis seedlings in this study indicate that as calcium concentration increases, the growth rate of Pinus tabuliformis seedling roots initially slows down and then accelerates, showing an increasing trend when calcium concentration reaches 400 mg·kg -1 .
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Pearson correlation analysis showed that the increase in yield was accompanied by the decrease in N 2 O emission, and there was a highly significant negative correlation between yield and N 2 O emission ( R = − 0.64), and there was a significant negative correlation between RE N and N 2 O emission ( R = − 0.37), with the higher the RE N , the lower the N 2 O emission.