Meanwhile, NM, MD and BPD groups were all significantly separated, and the difference was found to be highly significant by Adonis test ( p < 0.01).
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3.4 Effect of environmental factors on microbial communities In low density, bacterial community showed highly significant correlation with soil TP, AP, AK and SOM ( p < 0.01), and significant correlation with TK and AK ( p < 0.05), and fungal community showed highly significant correlation with soil EC, TP, AP, AK and SOM ( p < 0.01) ( Figure 7A ).
Highly significant differences are indicated by p < 0.01, significant differences by p < 0.05.
However, the survival rate of mice treated at 2 and 4 dpi did not reach statistical significance ( p < 0.01) when comparing the entire disease course, but did when comparing the last 18 days of the disease.
We selected only specific time intervals where the regression line between O 2 % air saturation and time was highly significant ( p < 0.01; R 2 > 0.90).
NK4A214_group showed a positive correlated with the numbers of total born and born alive ( p < 0.05), and a highly significant positive correlated with the numbers of healthy piglets ( p < 0.01).
Specifically, groups T2 and T3 exhibited a highly significant increase in NH 3 -N concentration compared to group T1 ( P < 0.01), with a linear increase in NH 3 -N levels corresponding to the escalating doses of LE.
Compared to CON, L-OEO exhibited a significant increase in the abundance of Bacteroidota ( p < 0.05) and a highly significant reduction in Firmicutes_A ( p < 0.01).
This study screened microorganisms and metabolites with a correlation C value of greater than 0.99 and a CCP < 0.01 and found that Christensenellaceae_R-7_group , Ruminiclostridium_9 , Zoogloea , and the metabolites avocadene 2-acetate, lysyl-asparagine, gamma-glutamyl cysteinylserine, and biocytin have extremely significant and strong correlations ( P < 0.01).
In contrast, when grown in SMB, Δ Tfsdh1 showed a highly significant increase in TfSDH1 activity, with levels 26.91% higher than Y32 ( p < 0.01) and 42.30% higher than Δ Tfsdh1 :: Tfsdh1 , respectively.
pH had a significant positive correlation with Shewanella ( p < 0.05); a negative correlation with Bradyrhizobium , unidentified_Alphaproteobacteria , and unidentified_Burkholderiaceae ( p < 0.05); and a highly significant negative correlation with Methylobacterium ( p < 0.01).
The relative abundance of Gammaproteobacteria -derived subclade E/2 showed a decreasing trend along the transection from inshore (3.04%) to offshore (0.36%), and negatively correlated with longitude ( r = −0.75, P < 0.01) (Figure S2 ).
The differences in both axial and secondary root lengths between the treatment and control groups were highly significant ( p < 0.01).
Evaluation of the explanatory power of cardiac structure and function indicators for gut microbiota variation Based on dbRDA analysis, significant associations were found between LVFWd, MWTd, and ET with gut microbiota ( p < 0.05), while LV_MASS_I showed a highly significant association with gut microbial communities ( p < 0.01).
Statistical significance was considered at p < 0.05, and highly significant differences were indicated at p < 0.01.
Non-metric multidimensional scaling (NMDS) revealed highly significant differences in β -diversity among varieties ( p < 0.01; Figure 2 ).
The waste reduction rate in the BSFL 1-10 group was 48.54%, also showing a highly significant difference ( p <0.01) compared to the control group.
polyphylla demonstrated a highly significant negative correlation with the abundance of Ascomycota ( R = −0.8815, P < 0.01), while showing significant positive correlations with Mortierellomycota ( R = 0.7120), Rozellomycota ( R = 0.6781), Aphelidiomycota ( R = 0.8001), and Monoblepharomycota ( R = 0.6809).
Regarding physicochemical parameters ( Supplementary Figure S2 ), temperature was negatively correlated with most genes, showing highly significant correlations with tetG , sul2 , aac(6′)-Ib-cr , and intI2 ( p < 0.01). pH correlated negatively with sul2 ( p < 0.01) and positively with tetW ( p < 0.05).
For the IM and FM groups, the difference was extremely significant ( P < 0.01) for the ACE and Chao 1 indexes, but not significant for the Simpson and Shannon indexes.
Difference-in-difference analysis of the two subgroups was visualized using violin plots and box plots, which showed significant intergroup differences ( Figures 1C , D ); ** denotes highly significant differences ( p < 0.01).
In the lower reaches region, the influence of water quality factors remained dominant, with TP, TN, and Turbidity showing highly significant effects ( p < 0.01).
Overall, PNSP belonging to PCV10 serotypes showed a decreasing trend, while PNSP associated with non-PCV10 serotypes showed an increasing trend ( Figure 4 ; p < 0.01).
The absolute LDA values of the five biomarkers were all greater than four, indicating highly significant differences ( p < 0.01), which may be the key biomarkers leading to differences in the bacterial community structure among the PF, CC, and SC, and different intensities of OC sample plots.
3.5 Serum levels of IL-6, TNF-α, TMAO, and hepatic TMAO in diarrhea with kidney-yang deficiency syndrome mice In Figure 4 , compared with the CN group, the CD group exhibited a significant increase in TNF-α ( p < 0.01) and a highly significant increase in IL-6 ( p < 0.01) in serum.