Barely Significant

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This is because microbial biomarkers are often based on specific fermentation patterns or relatively stable microbial community structures, and in the non-fermentor-driven fermentation dynamic environment, it is difficult to accurately capture markers with real indicative significance, and the dynamic changes of microbial communities represented by these markers become elusive, which can easily lead to mislead the subsequent analysis and conclusion derivation.
The early stage of vaccination induced a cellular immune response in pigs, as demonstrated by the serum concentrations of three types of cytokines (IL-2, IFN-γ, and TNF-α) in the vaccine-treated group, which exhibited an increasing trend at week 2 after the first immunization and then reverted to the similar levels to that of the control group at week 4 ( Figure 5B ).