Barely Significant
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“may be significant”

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In the literature

2 Therefore, the change in the analyte to IS peak area ratios may be significant enough to influence the accuracy of quantitation. 8 − 13 Several approaches have been recommended to overcome caveats associated with the use of d -labeled ISs. 12 − 17 Additional sample preparation steps, the use of shallow gradient profiles to achieve adequate separation of analytes from major matrix components eluting with the solvent front, application of atmospheric pressure chemical ionization (APCI) which is less prone to matrix effect than ESI, 2 or using other than d - (i.e., 13 C-, 15 N-, or 18 O-) labeled ISs are examples of these efforts.
The factors that significantly improved the reaction time, BSA blocking and EDTA, were carried over to a three-level Box–Behnken DOE study as well as Tris-HCl and Tween 20, which did not show as significant in the factorial experiment but were included in all runs, so their absence may be significant.