Barely Significant
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“extremely significant”

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These metabolites were mainly enriched in multiple alkaloid biosynthesis pathways, such as “Biosynthesis of alkaloids derived from histidine and purine” and “Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid.” In addition, the extremely significant differences in “Stilbenoid, diarylheptanoid and gingerol biosynthesis,” “Phenylpropanoid biosynthesis,” “Biosynthesis of plant hormones,” and “Purine metabolism” are also noteworthy (Fig. 4 c).
In this study, we constructed a metabolic set of the identified differential metabolites, and used KEGG enrichment analysis to clarify that interaction between the microorganisms on the surface of Italian ryegrass and the chemical components of Italian ryegrass in three regions had extremely significant effects on biosynthesis of other secondary metabolites (isoquinoline alkaloid biosynthesis, betalain biosynthesis), amino acid metabolism (alanine, aspartate and glutamate metabolism, tyrosine metabolism, tryptophan metabolism), carbohydrate metabolism (glyoxylate and dicarboxylate metabolism, citrate cycle, amino sugar and nucleotide sugar metabolism), membrane transport (ABC transporters), nucleotide metabolism (pyrimidine metabolism) and energy metabolism (nitrogen metabolism).
P < 0.01 was considered very significant, and P < 0.001 was extremely significant. 3 Results 3.1 Compositional analysis of CR-PLR decoction 3.1.1 Analysis of CR-PLR decoction by UPLC-Q-TOF-MS A total of 21 components were analyzed in the CR-PLR decoction in positive and negative ion mode, among which 11 components derived from CR and 10 components derived from PLR.
Correlation analysis was conducted on various parameter indicators of gene codons ( Figure 2 ), and the results showed that the correlation between GC 1 and GC 2 was extremely significant, but the correlation between GC 3 and GC 1 , GC 2 , and GC 12 was not significant, which indicated that the base compositions of the first and second positions of the gene codon of the encoding gene of the D. sinica plastid were relatively similar, while the base composition of the third position was relatively random and differed from the base compositions of the first and second positions.