Excerpts

A highly significant statistical analysis ( p = 1.14×10 −69 ), also in agreement with its experimental counterpart, further supported the evidence for gradient directed migration of the MV3 melanoma cells inside the virtual chamber.

Correlation between change in epigenetic marks and gene expression There was a highly significant correlation between changes in the repressive chromatin mark H3K9me3 and changes in DNA methylation (rho 0.136, p= 1.7×10 −69 ).

Furthermore, we determined that approximately one-third of the Nup98-bound genes derived from CHIP Seq analysis in Drosophila cells that have mouse orthologs were FoxK1 bound and regulated ( 30 , 39 ), which is highly significant ( P = 1.85754E–69) (see Fig.

In the GSE14520 cohort, risk scores showed a trending stage-dependent increase from nontumor tissues to early-stage HCC (BCLC 0/A) and more advanced HCC stages (BCLC B–C), with highly significant differences across all groups (overall p =1.86×10 −69 ; Fig. 5A ).

The ChIP-seq targets of Cbf11 and Cbf12, on the other hand, showed a highly significant overlap (71 shared genes, p = 4 × 10 −69 ).

This is a highly significant overlap (hypergeometric test in R, p = 6.079e-69) ( Fig 3 ).

As shown in Figure 1 A, breast tumors demonstrated significant overexpression of EDB-FN (median 36.319 transcripts per million (TPM)), compared to normal breast tissues (median 2.410 TPM), translating to an extremely significant log 2 fold increase of 3.452 (adj p value = 6.57 × 10 −69 ) in breast cancer over normal tissues.

The overlaps with plasma were 47 (T1∩plasma) and 54 (M1∩plasma), both highly significant after Bonferroni correction (Fisher’s exact test p < 10−68 and p < 10−74; Supplementary Fig. 2a ), supporting the reliability of the plasma-derived ground truth.

We detected interspecies positive correlations as high as 0.36 ( Figure 2 A), which is extremely significant ( p -value = 1.52 × 10 −68 , Figure 2 B).

This difference is highly significant ( t -test, P = 2.15 × 10 − 68 ).

The regression model including these four significant factors was moderately predictive of matrix-like volume (R 2 = 0.32) but highly significant (F (7, 844) = 57.8, p = 1.2 × 10 –67 ).

The contingency table comparing the results from the two classification algorithms yielded a highly significant level of association based on Fisher’s exact test ( P = 2.15 × 10 −67 ).

The difference in free energy values between genes with decreased TE in Silvestrol and insensitive genes was highly significant ( P =2.53 × 10 -67 ) and a difference in distribution of ∆G values from genes with increased TE was also notable compared with the insensitive pool ( P =4.25 × 10 -7 ) (Figure 2 a).

This overlap is highly significant (p = 2.8E-67) ( Table S2 ).

A scatter plot of gene significance (GS) against module membership (MM) for genes in the blue module indicated a highly significant positive correlation (correlation coefficient = 0.77, P =9.2×10 −67 ; Figure 2D ), implying that these genes may play central roles in ferroptosis regulation.

The obtained test statistic demonstrated a highly significant variation among algorithms (χ2 = 361.47, df = 17, p = 2.02 × 10 − 66), confirming that the null hypothesis of equivalent performance can be rejected at the 0.05 significance level.

Of the signs and symptoms, 269 were significantly enriched in specific diagnoses, of which 148 were also a priori defined to be of diagnostic importance, a highly significant enrichment (χ 2 = 295.96, P = 2.5 × 10 −66 ).

Analysis of gene expression data from 499 PCa patients (TCGA, Firehose legacy project) revealed a highly significant positive Pearson’s correlation ( p = 4.68e − 66 ) between AR and DHX9 expression (Fig. 4 A).

Of the 9555 SRF regions from Triform, 124 regions overlapped with MER57 repeats (with an average overlap of 91%), and 117 of these contained at least two significant binding sites for TFs (from the CisFinder analysis), which is highly significant ( p = 1.2 × 10 -65 according to a Fisher exact test).

Gene ontology (GO) enrichment analysis displayed a highly significant over-representation of the “RNA-binding” molecular function category (GO:0003723), with an enrichment score of 147.76, p = 6.77 × 10 −65 , and FDR step up = 1.47 × 10 −60 for 6A1, and an enrichment score of 98.44, p = 1.77 × 10 −43 , and FDR step up = 3.84 × 10 −39 for clone 3B2 ( Figure 6 ).

The enrichment score generated for the EIF2 canonical pathway enrichment was extremely significant ( P -value = 1×10 −64 ) and the combination of these observations indicates that the gene-list was both predictable (valid with extensive literature) and statistically very robust ( Figure S2 ).

We find a highly significant map location at position 27.75 to 27.81 Mb on chromosome 9 ( P = 1.51 × 10 −64 ; Fig. 2, A and B ) and no evidence of statistical inflation (fig.

Nevertheless, highly significant differences were seen in the cellular morphology ( p = 1.97 × 10 −64 ).

COL6A3, COL1A1, COL3A1, THBS2, COL5A1, and LAMC1 all exhibited significant upregulation with highly significant p-values (e.g., COL6A3, p = 2.89 e-64 ; COL1A1, p = 9.21 e-90 ) ( Figure 7A ).

The G+C content of putative introns ( Table 5 ) shows a highly significant (p = 3.3E −64 ) difference to that of exonic regions in accord with the general pattern in plants (higher GC in coding regions) and consistent with a parallel analysis of retained introns identified from genomic annotation in the A. thaliana data.