Barely Significant

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a decreasing trendP < 0.051.0× alphagold
Compared to the Con group, the ingested nitrogen of the piglets in the Rha group, Lac group, and mix group was significantly increased ( P < 0.05), fecal nitrogen, urinary nitrogen, and total nitrogen emissions showed a decreasing trend, the apparent nitrogen digestibility of Rha and Lac groups tended to increase; the apparent nitrogen digestibility of the mix group increased by 1.88% ( P < 0.05), and the nitrogen deposition rate increased respectively increased by 4.17, 4.49, and 5.65% ( P < 0.05), and the nitrogen utilization rate increased by 2.82, 3.23, and 3.70%, respectively ( P < 0.05). 3.2.
highly significantp < 0.051.0× alphaqualifiedgold
Combined NMDS and MRPP analyses revealed a highly significant difference in community structure between the L group and the other two groups ( p < 0.05) (ANOSIM, p = 0.008 and 0.028; MRPP, p = 0.009 and 0.015; Table 6 ). 3.4 Functional predictions in the fecal microbiota of female donkeys across different reproductive cycles We used PICRUSt to predict the functions in the fecal microbiota of female donkeys across different reproductive cycles and evaluated the accuracy of these predictions using the Nearest Sequenced Taxon Index (NSTI) values.
an increasing trendP < 0.051.0× alphagold
The 50% rainfall enhancement treatment significantly increased soil moisture, and the total carbon content showed an increasing trend with the increase in precipitation ( P < 0.05). pH was the most important explanatory factor for community structure, while total nitrogen content was the key explanatory factor for community diversity.
a decreasing trendp < 0.051.0× alphagold
Relative to bare tailings, BSCs samples exhibited elevated concentrations of Fe, Cd, and Mn at both H and L groups, Zn at L group, and Al at H group, whereas Cu at H group showed a decreasing trend ( Supplementary Table S1 ; Tukey’s HSD, p < 0.05).
a decreasing trendp < 0.051.0× alphagold
During the 3-year period, the resistance rate to piperacillin-tazobactam initially declined but then increased ( p < 0.05), while resistance to ceftazidime-avibactam, aztreonam, imipenem, ciprofloxacin, levofloxacin, and amikacin showed a decreasing trend ( p < 0.05).
highly significantp < 0.051.0× alphaqualifiedgold
Species differences in different groups The multiple groups’ significant difference test illustrated in Figure 4A reveals that Erysipelotrichaceae_UCG-003 , Lachnospiraceae_UCG-004 , Tyzzerella , and other genera, demonstrated statistically significant or highly significant differences in average relative abundance across the five groups ( p < 0.05, p < 0.01, p < 0.0001).
highly significantp < 0.051.0× alphaqualifiedgold
We performed Pearson correlation analysis of MP degradation gene profiles with genus-level microbes and selected highly significant and highly correlated (0.7 < |r| < 1, p < 0.05 ) relationships to demonstrate co-occurring networks, which indicated that microbes may be potential hosts for significantly enriched MP degradation gene profiles ( Figure 4 and Supplementary Table S2 ).
a decreasing trendP < 0.051.0× alphagold
an increasing trendP < 0.051.0× alphagold
Many genera from Firmicutes exhibited an increasing trend ( P < 0.05), including Vagococcus , Roseburia , Enterococcus , Facklamia , Lactobacillus , Faecalibacterium , unclassified Anaerostipes , Lachnobacterium , Coprococcus , Anaerovibrio , Clostridium _ sensu _ stricto , Faecalicoccus , and Staphylococcus , while many genera others exhibited a decreasing trend ( P < 0.05), including Sporosarcina , Oscillibacter , Peptoniphilus , Anaerobium , unclassified Sporobacter , Amphibacillus , Faecalicatena , and Butyrivibrio ( Figure 3 and Supplementary Data 2 ).
highly significantp < 0.051.0× alphaqualifiedgold
In the time series of Cr stress, the phylum Actinomycetes ( p < 0.01) and Chloroflexi ( p < 0.05) both displayed highly significant increases followed by decreases, while Firmicutes ( p < 0.05) and Myxococcota ( p < 0.01) displayed significant decreases; the phyla Proteobacteria ( p < 0.01) and Gemmatimonadota ( p < 0.01) both displayed highly significant decreases followed by increases.
an increasing trendp < 0.051.0× alphagold
The rates of Klebsiella pneumoniae resistance to piperacillin/tazobactam, ticacillin/clavulanate, cefazolin, cefuroxime sodium, cefotaxime, ceftriaxone, ceftazidime, cefotetan, amicacin, tobramycin, gentamicin, norfloxacin, and ciprofloxacin showed an increasing trend ( p < 0.05).