When analyzing the relationship between microbial communities and soil chemical properties, Mantel's tests reveal a significant correlation between bacterial communities and MBC, MBN ( P < 0.05), and a highly significant correlation with pH, SOM, SOC, TN, TP, AN, OP, AK ( P < 0.01).
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A significant trend toward increased number of resistant strains over time was observed only in GC_91 isolates ( r 2 = 0.607; p < 0.01; Table 4 ).
However, the two combinations, NP + P and NP + P + L of SG10, caused a highly significant increase in FLuc activity compared with the native LaSota strain ( p < 0.01) ( Figure 6A ).
Comparing between groups, the virus titers obtained from the youngest ducks were highest, and virus titers in heart and lung were 10 1.5 –10 2.3 higher than those of the other two groups, with highly significant difference ( P < 0.01, Figure 3B ).
The absolute LDA values of the five biomarkers were all greater than four, indicating highly significant differences ( p < 0.01), which may be the key biomarkers leading to differences in the bacterial community structure among the PF, CC, and SC, and different intensities of OC sample plots.
However, both pH and SEC showed highly significant negative correlations with all other soil physicochemical properties and vegetation characteristics ( p < 0.01).
Difference-in-difference analysis of the two subgroups was visualized using violin plots and box plots, which showed significant intergroup differences ( Figures 1C , D ); ** denotes highly significant differences ( p < 0.01).
In the lower reaches region, the influence of water quality factors remained dominant, with TP, TN, and Turbidity showing highly significant effects ( p < 0.01).
Synergy (FIC Index ≤0.5) was found in 21/32 strains and was highly significant ( p < 0.01) in strains where resistance is expressed only in presence of erythromycin.
The abundance of Gibellulopsis showed a highly significant positive correlation with soil pH ( P < 0.01).
IL-1β expression tended to be higher at 3 dpi in Groups 2 and 3, whereas it showed a decreasing trend over time in the other groups ( p < 0.01) ( Figure 6D ).
The Shannon index showed a highly significant negative correlation with soil pH ( r = −0.860, P < 0.01) and a significant positive correlation with MBC ( r = 0.675, P < 0.05), along with a highly significant positive correlation with MBN ( r = 0.946, P < 0.01).
Statistical significance was denoted as follows: * indicates a statistically significant difference ( p < 0.05), ** indicates a highly significant difference ( p < 0.01), and *** indicates an extremely significant difference ( p < 0.001).
For hydroxyl radical (-OH) scavenging capacity, the WIS32 fermentation supernatant demonstrated a highly significant advantage, with a clearance rate as high as 37.26% ± 2.15%, far exceeding that of LFG89 (7.23% ± 0.95%, p < 0.01).
Relationship between soil microbial communities and physicochemical properties/enzyme activities Correlation analysis between the top 20 genera and physicochemical properties revealed the following: for bacteria ( Figure 6A ), AK showed a highly significant positive correlation with Blastococcus ( p < 0.01) and significant positive correlations with Rubrobacter, unclassified_c_Actinobacteria, Pseudonocardia, norank_f_67-14, Nocardioides , and Skermanella ( p < 0.05), but a significant negative correlation with Sphingomonas .
In the genus-level heatmap ( Figure 4B ), marked by red boxes, Haemophilus and Fusobacterium exhibited high abundance (red) in the non-tumor group (S.N, bottom row) but were significantly decreased (blue) in the lung cancer group (S.L, top row), with statistical tests showing highly significant differences ( P < 0.01, dark red diamonds).
Specifically, groups T2 and T3 exhibited a highly significant increase in NH 3 -N concentration compared to group T1 ( P < 0.01), with a linear increase in NH 3 -N levels corresponding to the escalating doses of LE.
Differences were considered statistically significant with ∗ P < 0.05 and highly significant with ∗∗ P < 0.01 and ∗∗∗ P < 0.001.
penetrans populations was highly significant ( p < 0.01) and there was a positive correlation between the degree of female bias and the prevalence of Wolbachia .
For example, in a study conducted to evaluate the carvacrol-erythromycin synergistic effect against erythromycin-resistant Group A Streptococci, highly significant synergy (FIC Index ≤ 0.5, p < 0.01) was detected in 21 out of 32 strains ( Magi et al., 2015 ).
The relative abundance of Gammaproteobacteria -derived subclade E/2 showed a decreasing trend along the transection from inshore (3.04%) to offshore (0.36%), and negatively correlated with longitude ( r = −0.75, P < 0.01) (Figure S2 ).
We selected only specific time intervals where the regression line between O 2 % air saturation and time was highly significant ( p < 0.01; R 2 > 0.90).
A * P < 0.05 was considered statistically significant and highly significant when ** P < 0.01 and *** P < 0.001 (two-tailed tested).
The differences in both axial and secondary root lengths between the treatment and control groups were highly significant ( p < 0.01).
For the IM and FM groups, the difference was extremely significant ( P < 0.01) for the ACE and Chao 1 indexes, but not significant for the Simpson and Shannon indexes.