Barely Significant

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The statistical significance was analyzed by the unpaired Student’s t -test and one-way ANOVA (*: p < 0.05; **: p < 0.01; ***: p < 0.001); differences with p < 0.05 were considered significant, those with p < 0.01 were considered very significant, and those with p < 0.001 were considered extremely significant. 3 Results 3.1 Successful construction of E. coli BL21 (pET-28a- bla NDM–5 ) Due to the complex genetic background of clinically isolated bla NDM–5 -positive E. coli , the plasmid pET-28a containing the bla NDM–5 fragment was transformed into wild-type E. coli BL21 to exclude potential interference.
However, even if we recorded an increase in PCBs in the 2019 samples, compared to those of 2018 (possibly in dependence on the different sampling sites, i.e., the inner and outer bay), this result gives rise to hope that the banning of the production of PCBs in the 1970s has determined a decreasing trend in their transport and bioaccumulation at higher latitudes.