The comparisons among the groups were done using either Wilcoxon rank test or two-way ANOVA testing for effects of genotype and surgery independently, and where it was suggested by the plots and data, we also tested possible interactions between these factors (i.e., whether the effect of genotype was different after surgery to before), followed by Welch two-sample t -test for the Adm AM+/Δ mice with or without AM supplement, with a two-tailed significance set at the 0.05 levels and marginal significance set at the 0.1 level.
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